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dc.contributor.author | Maciel, Joyce Belentani de Souza | - |
dc.date.available | 2021-01-10 | - |
dc.date.available | 2022-01-10T13:09:59Z | - |
dc.date.issued | 2019-11-13 | - |
dc.identifier.citation | MACIEL, Joyce Belentani de Souza. Identificação e caracterização de bactérias com potencial para controle biológico. 2019. 35 f. TCC (Graduação em Ciências Biológicas) - Universidade do Estado do Amazonas, Manaus. | pt_BR |
dc.identifier.uri | http://repositorioinstitucional.uea.edu.br//handle/riuea/3631 | - |
dc.description.abstract | Biological control through the use of microorganisms is an alternative to reduce the use of pesticides in the control of plant pathogens, which affect plants of agricultural interest. In this context, the objective of this work was to identify and quantify the inhibition potential of previously selected bacteria against phytopathogenic fungi. Eight microorganisms were evaluated: three endophytic isolates obtained from guaranazeiro roots (PcA1, PcA2, PcA3)) and five isolates from Madeira River sediments, from these four actinobacteria (MAD 24, MAD 42, MAD 51 and MAD 189). bacteria (MAD 207)). These were identified by morphomolecular analysis based on scanning electron microscopy and 16S rDNA region sequencing data. Inhibition potential (PIC) against four phytopathogens: Colletotricum siamense, Pseudopestalotiopsis gilvanis sp. nov., Neopestalotiopsis formicarum and Fusarium decemcellulare, were obtained in vitro using the double culture methodology in BDA medium. The mean percentage inhibition (PIC) after 10 days ranged from 46% to 74% depending on the bacteria tested. The isolates PcA1, PcA2, PcA3 and MAD207 performed better against F. decemcellulare (71% ± 2.8, 64% ± 1.7, 72 ± 4.5 and 78% ± 1, respectively). The MAD24 isolate showed better performance against N. formicarum (72% ± 0.5), MAD42 and MAD51 against C. siamense (72% ± 0.5; 74% ± 2.4), and MAD189 against N. formicarum (69% ± 0.5). PcA1, PcA2, PcA3 and MAD207 isolates were identified, based on sequence analysis of the 16S rRNA gene, as members of the Bacillus genus, which were phylogenetically related to B. amyloliconefaciens, B. subtilis, B. nakamurai and B. velezensis respectively. . Scanning electron microscopy data revealed different morphology for each isolate, suggesting that they belong to different species. Isolates MAD24, MAD42, MAD 51 and MAD189 were identified as members of the genus Streptomyces. Samples MAD189 and MAD42 were more phylogenetically related to each other and formed a separate clade, MAD51 showed low phylogenetic relationships with the analyzed species and MAD24 was grouped in the constraricanus clade, S. griseofuscusmurinus. The morphological data corroborate the molecular inferences and differences in size and shape were identified in all isolates.. Keywords: Actinobacteria; Antimicrobials; Biological control. | pt_BR |
dc.language | por | pt_BR |
dc.publisher | Universidade do Estado do Amazonas | pt_BR |
dc.rights | Acesso Aberto | pt_BR |
dc.subject | Actinobactérias | pt_BR |
dc.subject | Antimicrobianos | pt_BR |
dc.subject | Controle biológico | pt_BR |
dc.subject | Biological control | pt_BR |
dc.subject | Antimicrobials | pt_BR |
dc.title | Identificação e caracterização de bactérias com potencial para controle biológico | pt_BR |
dc.title.alternative | Identification and characterization of bacteria with potential for biological control | pt_BR |
dc.type | Trabalho de Conclusão de Curso | pt_BR |
dc.date.accessioned | 2022-01-10T13:09:59Z | - |
dc.contributor.advisor-co1 | Silva, Gilvan Ferreira da | - |
dc.contributor.advisor-co1Lattes | http://lattes.cnpq.br/1000535673605322 | pt_BR |
dc.contributor.advisor1 | Batista, Ieda Hortêncio | - |
dc.contributor.advisor1Lattes | http://lattes.cnpq.br/5290529604475961 | pt_BR |
dc.contributor.referee1 | Santos, Josenilda Carlos dos | - |
dc.contributor.referee1Lattes | http://lattes.cnpq.br/1101479829656159 | pt_BR |
dc.contributor.referee2 | Lima Júnior, Raimundo Sousa | - |
dc.contributor.referee2Lattes | http://lattes.cnpq.br/2055426262014670 | pt_BR |
dc.creator.Lattes | http://lattes.cnpq.br/5908436186574161 | pt_BR |
dc.description.resumo | O controle biológico por meio do uso de microrganismos é uma alternativa para diminuir o uso de defensivos químicos no controle de fitopatógenos, que afetam plantas de interesse agrícola. Neste contexto, o objetivo do trabalho foi identificar e quantificar o potencial de inibição de bactérias previamente selecionadas contra fungos fitopatogênicos. Foram avaliados oito microrganismos, sendo três isolados endofíticos obtidos de raízes do guaranazeiro (PcA1, PcA2, PcA3) e cinco isoladas de sedimentos do Rio Madeira (MAD 24, MAD 42, MAD 51, MAD 189 e MAD 207). Estes foram identificados por meio de análise morfomolecular, com base em dados de microscopia eletrônica de varredura e sequenciamento da região 16S rDNA. O potencial de inibição (PIC) contra quatro fitopatógenos: Colletotricum siamense, Pseudopestalotiopsis gilvanis sp. nov., Neopestalotiopsis formicarum e Fusarium decemcellulare, foi obtido in vitro por meio da metodologia de cultura dupla, em meio BDA. A média do percentual de inibição (PIC) após 10 dias variou de 46% a 74% dependendo da bactéria testada. Os isolados PcA1, PcA2, PcA3 e MAD207 apresentaram melhor desempenho contra F. decemcellulare (71%±2.8, 64%±1.7, 72±4.5 e 78%±1, respectivamente). O isolado MAD24 apresentou melhor desempenho contra N. formicarum (72% ±0.5), MAD42 e o MAD51 contra C. siamense (72% ±0.5; 74% ±2.4), e MAD189 contra N. formicarum (69% ±0.5). Os isolados PcA1, PcA2, PcA3 e MAD207 foram identificados, com base na análise de sequência região 16S, como membros do gênero Bacillus, estes foram relacionados filogeneticamente a B. amyloliquenfaciens, B. subtilis, B. nakamurai e B. velezensis respectivamente. Dados de microscopia eletrônica de varredura revelaram morfologia diferente para cada isolado, o que sugere que estes pertencem a diferentes espécies. Os Isolados MAD24, MAD42, MAD 51 e MAD189 foram identificados como membros do gênero Streptomyces. As amostras MAD189 e MAD42 foram filogeneticamente mais relacionadas entre si e formaram um clado separado, MAD51 mostrou baixa relações filogenéticas com as espécies analisadas e MAD24 foi agrupado no clado de S. costaricanus, S. griseofuscusmurinus. Os dados morfológicos corroboram com as inferências moleculares e diferenças de tamanho e forma foram identificadas em todos os isolados. Palavras-chave: Actinobactérias; Antimicrobianos; Controle biológico. | pt_BR |
dc.publisher.country | Brasil | pt_BR |
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PloS one, v. 12, n. 1, p. e0170557, 2017. | pt_BR |
dc.subject.cnpq | Ciências Biológicas | pt_BR |
dc.subject.cnpq | Biologia molecular | pt_BR |
dc.publisher.initials | UEA | pt_BR |
Aparece nas coleções: | ENS - Trabalho de Conclusão de Curso de Graduação |
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